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Our Team


Jen Lochren-Loureiro, Owner of Healing Waters of Raleigh, is a child of God, wife and mother of two beautiful daughters. She is an I-ACT Certified Colon Hydrotherapist and Licensed Esthetician.


Jen’s passion for Colon Hydrotherapy began during a time when she was experiencing many life stresses which brought on many stomach issues. She had always struggled with anxiety, headaches, and gut related problems. After going to countless doctors with no solutions, Jen started to take her health into her own hands. She researched ways to combat this ongoing dilemma, and on her journey, she came across enemas and Colon Hydrotherapy.


Her personal positive experience with Colon Hydrotherapy, which alleviated multiple issues, led her to recognize the benefits of this therapy and the importance of maintaining a healthy digestive tract. It was evident that her diverse health problems had their origins in her gut and could effectively be addressed with colon cleansing. Knowing this information and seeing how Colon Hydrotherapy has changed her life, Jen knew that she wanted to get certified, so she can change others' lives as well!


Prior to training, Jen received a Bachelor’s Degree in Science/Business in 2003 and she earned a license in Esthetics in 2004. Jen is an empath who is caring, sensitive to people’s needs, and very passionate about her work. She is dedicated to helping others on their journey to optimum health and wellness.


Darlene Holloway, ND, CT, LMBT, SIT brings over 44 years of expertise in the field of holistic health, particularly Colon Hydrotherapy to Healing Waters of Raleigh.

Darlene is a Naturopathic Doctor, Advanced Colon Hydrotherapist , and an Advanced Muscle Therapist. She has devoted over 40 years to teaching and serving others by integrating a variety of non-traditional preventive, restorative, and health maintenance therapies. She is passionate about the work she offers to her clients and students. She has opened and maintained two full time clinics and schools.

Darlene is regarded as one of the leaders in the field of Colon Hydrotherapy by clients, schools, and fellow therapists. She has received many of the highest awards in the field, including Colon Hydrotherapist of the Year by the International Association of Colon Hydrotherapy (I-ACT), and she has served as a board member of the association.

As a Licensed Massage and Colon Therapist with the State of Florida, she helped rewrite the curriculum for the State of Florida Licensing Board for Massage and Colon Therapy. She was voted President of the North Carolina Colon Hydrotherapy Safety Association. She continues to build on her knowledge, and today holds the highest level of certification as an instructor and therapist with I-ACT and the National Board Of Colon Hydrotherapy.

Darlene loves the outdoors. Among her many interests have been windsurfing, raising horses, and gardening. She and her husband have been married for over 48 years, and have been blessed with two children and three grandchildren.

Crystalyn outside.jpeg

Crystalyn Roberts is a dynamic individual who has dedicated her career to promoting wellness and holistic health practices. As the Office Manager at Healing Waters of Raleigh, Crystalyn brings a wealth of experience and a genuine passion for helping others achieve optimal health and well-being.

Crystalyn's journey into the wellness field began with a deep-seated interest in promoting oral health and overall wellness. Armed with a Bachelor's Degree in Dental Hygiene from UNC-Chapel Hill, she embarked on a career as a Licensed Dental Hygienist, accumulating over 20 years of experience in the field.

Growing up with digestive health issues during her childhood, Crystalyn developed a keen interest in exploring holistic solutions to improve her well-being. She discovered the transformative power of plant-based solutions and Colon Hydrotherapy in alleviating digestive issues and enhancing overall health. Inspired by her personal experiences, Crystalyn has become a staunch advocate for holistic approaches to wellness, firmly believing in the body's innate ability to heal itself when provided with the right tools and support.

Beyond her role as an Office Manager, Crystalyn is a devoted boymom of four and a dedicated business owner. She finds immense fulfillment in serving her community, both locally and abroad, through various outreach initiatives and volunteer work. Her commitment to making a difference in the lives of others extends far beyond the confines of her professional career, embodying her unwavering dedication to promoting wellness and holistic living. Whether it's through phone calls, emails, or text messages, Crystalyn ensures that every interaction with potential clients is informative, supportive, and reassuring, laying the groundwork for a positive and empowering wellness journey


Melissa is a native of Eastern NC, a graduate of East Carolina University and a new transplant to Raleigh. She comes to us with over 20 years in post acute care.


Her journey to Colon Hydrotherapy started as a client here, at Healing Waters of Raleigh. What started as a journey to improve her overall well being turned into a new passion and career as a Colon Hydrotherapist. In her free time, Melissa enjoys spending time with her family & friends, live music events and is always searching for her next DIY project!

I-ACT LOGO - Member

For more information, contact I-ACT at: I-ACT • PO Box 461285 San Antonio, TX 78246-1285 P: (210) 366-2888 • F: (210) 366-2999 W: www.i-actorg • E:



Modern colon hyrdrotherapy equipment is manufactured through compliance with strict federal guidelines that dictate rigorous accountability. Disposable, single-use rectal tubes and/or speculae are highly recommended. I-ACT standards require the facility to be 
immaculately clean. 

"He's the best physician who knows the worthlessness of most medicines."

Benjamin Franklin 

10 Ways to Improve Digestion

I-ACT Mission Statement

The mission of the International Association for Colon Hydrotherapy (I-ACT) is to heighten the awareness of the colon hydrotherapy profession, ensure continuing and progressive education within the field, and implement professionalism beyond reproach. 

Hippocrates claimed that chronic disease came from autointoxication, i.e. self poisoning due to constipation. 

colon Hydrotherapy

Colon Hydrotherapy

Today’s diet consists in great part of refined, processed foods, high in saturated fats and low in fiber. Eating these foods, overeating, ignoring the call the eliminate stress, allergies, medications, lack of exercise and environmental toxins can contribute to over-acidity in the body, sluggish, poor digestion, respiratory and skin problems and poor colon health.

The colon contains the largest concentration of bacteria in the body. Waste matter that stagnates alters the proportion of healthy vs. harmful bacteria disrupting the balance of the healthy microorganisms that are essential to the health of the colon and the body in general. Symptoms such as constipation, diarrhea, IBS, gas and bloating, body odor, fatigue and bad breath may occur. Regular colonics help to keep your digestive system, kidneys and liver functioning properly which helps your body to cleanse and eliminate regularly on its own.

During a colon hydrotherapy (colonic) session, a narrow disposable speculum is partially inserted with a round-tipped applicator approximately 2-3 inches into the rectum. Filtered, temperature controlled water is introduced into the colon and released several times during a session. The Colon Hydrotherapist is always present; attentive to the client (who is covered at all times) and closely monitoring the temperature and pressure-sensitive flow of water. During an outflow, after introducing water into the colon,  water and waste material travels from the disposable outflow tube through a glass viewing tube within the wall unit and ultimately into the drainage system. During an outflow, the abdomen is gently manipulated, encouraging an easier and more complete release. This cycle of filling and emptying is repeated several times during the session. Colonics can cleanse the entire colon using filtered water and without the use of drugs or chemicals. The device is a “closed system” which affords a comfortable procedure while maintaining the dignity of the patient. There is no open evacuation and no offensive odors. It is therapist assisted for effectiveness, comfort and most importantly, safety. The device comes equipped with a built-in water treatment system. This patented system will remove particulate matter, reduce the amount of chlorine and, with the use of Ultraviolet light, destroy 99.9% of bacteria and viruses that may be in the water.

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